development of photobioreactors and approach for large-scale production of marine algae chrysotila and nannochloropsis in inland environment
abstract
due to energy, environment, and food problems, research on microalgae is increasingly
gaining attention. microalgae can utilize photosynthesis to synthesize and accumulate
various valuable bioproducts such as lipids, proteins, polysaccharides, pigments, and can
also absorb and fix carbon dioxide. therefore, efficiently, and cost-effectively cultivating
specific algae species has become a crucial goal. however, various challenges hinder the
development of algae during cultivation. this study focuses on the indoor and outdoor
cultivation of two marine microalgae, chrysotila (pleurochrysis) dentata and
nannochloropsis oceanica.
for the cultivation of chrysotila (pleurochrysis) dentata, optimal indoor cultivation
conditions were determined with a light cycle of 18 hours light / 6 hours dark, ph of 8.5,
and salt concentration of 2.5 %. when chrysotila (pleurochrysis) dentata formed a
symbiotic system with the bacterium nitratireductor aquibiodomus, a ratio of 8:2 (algae to
bacteria) resulted in optimal accumulation of dry weight, chlorophyll α, and calcium
carbonate, while ratios 6:4 was more suitable for lipid accumulation. in the experiment on
the impact of microplastics polyethylene terephthalate (pet) on chrysotila (pleurochrysis)
dentata, it was observed that low concentrations of plastic (5-15 mg/l) promoted algae
growth. however, high concentrations of microplastics (20-50 mg/l) slowed algal growth. [...]