a high throughput screening and characterization of laccase-producing bacterium serratia quinivorans aorb19 that exhibits lignin degradation traits, dye decolorization efficiency, and enhanced laccase production in biomass
abstract
laccase (ec 1.10.3.2) assumes a vital role in the degradation of lignin by utilizing oxygen as an oxidant
and catalyzing bond cleavage in both phenolic and nonphenolic lignin model compounds. however, their
industrial application is limited due to reduced enzymatic activity and lower tolerance to extreme
conditions in most laccases isolated from microorganisms. to address these challenges, bioprospecting
for strains harboring novel laccases with enhanced activity and versatile properties is paramount. natural
biodegradation processes offer promises for lignin degradation, and rapid screening methods aid in
isolating microorganisms secreting extracellular lignin-degrading enzymes, including laccases. in our
study, we developed a novel high-throughput screening process to isolate a promising lignin-degrading
bacterium from decomposed wood samples. whole-genome analysis and lc-uv (liquid
chromatography-ultraviolet detection) analysis was employed to identify the species and assess its
lignin-degrading traits. this bacterium exhibited significant extracellular laccase production and was
biochemically characterized. moreover, the potential of this bacterium in bioremediation, specifically in
the decolorization of two major dyes, was probed. additionally, the laccase secretion capabilities of the
bacterial strain were assessed by utilizing low-cost industrial wastes from the canadian agro-industries. [...]