characterization of novel cellulase and glucose isomerase-producing bacteria and optimization of the enzyme production conditions and their potential applications in environment and industry
abstract
cellulases and glucose isomerases are vital enzymes in converting cellulose into fructose.
cellulases catalyze cellulose conversion to glucose, while glucose isomerase catalyzes the
reversible isomerization of glucose to fructose. there is a growing interest lately in producing
bio-based chemicals and materials from fructose. soil bacteria produce these enzymes. the
characterization of bacteria for enzyme saccharification of biomass is essential for fructose
production and reducing the time and cost of current bioconversion processes. from this
perspective, we characterized novel cellulase and glucose isomerase-producing bacteria from soil
samples and optimize their enzyme production. coculturing and whole-cell immobilization for
glucose isomerase and bacterial resistance to environmental factors were also investigated.
six bacterial strains, paenarthrobacter sp. mkal1, hymenobacter sp. mkal2,
mycobacterium sp. mkal3, stenotrophomonas sp. mkal4, chryseobacterium sp. mkal5 and
bacillus sp. mkal6 were isolated from mixture soil samples collected at kingfisher lake and the
university of manitoba campus and identified using 16s rrna gene sequence analysis. using
plate assay techniques, these strains were selected for cellulase and glucose isomerase production
based on the clearance zone appearance. these strains displayed various morphological and
biochemical characteristics. [...]