effect of carbon-starvation on the survival of and pnp degradation by a moraxella strain
abstract
the effect of carbon-starvation on the stress resistance responses of a p-nitrophenol
degrading moraxella strain was examined in both, buffer and river water
samples. the moraxella strain showed optimal stress resistance responses in a minimal
salt buffer when carbon-starved for 1-2 days. in the buffer system, the 1- and 2-day
carbon-starved moraxella cultures survived about 150-, 200- and 100-foid better than the
non-starved cultures when exposed to 43.5°c, 2.7m naci and 500 " h2o2 for 4 hours,
respectively. a green fluorescent protein gene- labelled derivative of the moraxella
strain was used to examine the stress resistance responses of the bacterium in natural
river water microcosms. the carbon-starved gfp-labelled moraxella strain also showed
stress resistant responses against heat, osmotic and oxidative stresses in the river water
samples. despite the stress tolerant capability of the carbon-starved gfp-labelled
moraxella cells, they did not exhibit any survival advantage over their non-starved
counterparts when inoculated into river water microcosms and incubated at 10° or 22°c
for fourteen days.
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- retrospective theses [1604]