physiology of development, germination and ubiquitination in pea seeds
abstract
the development of entire seeds and axes of pisum sativum l. cv. spring
was followed between 18 and 47 days after flowering, in terms of changes in fresh
weight, dry weight, water content, protein level and chlorophyll content. the seed
developed normally, but somewhat slower than suggested in other reports. fresh
immature seeds and axes placed in culture were able to germinate after a lag phase
which varied depending upon the time of detachment of the seed from the legume
pod and the axis from the seed. the seeds from all stages tested produced a viable
seedling. except for the least mature, the axes germinated fully, but grew only
slightly. root growth was particularly impaired.
ubiquitin conjugates were identified by sodium dodecyl sulfate polyacrylamide
gel electrophoresis (sds-page), western blotting and labelling with rabbit anti-ubiquitin
followed by 125 i goat antirabbit-igg. ubiquitinated proteins were detected
in seeds during development and germination, but not in dry mature seeds. only
free ubiquitin was observed in extract from dry seed. a correlation between higher
concentration of atp in the seed, and presence of ubiquitin conjugates was
observed. these results demonstrate that pea seed can contain both unconjugated
and conjugated ubiquitin.
collections
- retrospective theses [1604]