olympus fv300 confocal microscope
features:
- digital resolution: 256x 256 to 2048x2048. standard acquisition is 1024 x 1024
- grey scale: 0-4095
2 channels- z and time slices
equipment:
- olympus fluoview fv300 with fluoview 4.0 software
- bx51 microscope: bx51trf bx51 stand, trans/fluorescence, trinoc observ tube
- fv-ltv-u laser encl with video port
- lasers: argon (10 mw, force air cooled, blue 488nm), hene green(1mw, 543nm) and hene red (10mw 633 nm)
- fv300/500-laser combiner
- fv3-psu scan unit (fv-su302): . with pci/f card,
- 2 galvanometer scanner mirrors, confocal aperatures 60,100,150,200 and 300 um, pmt detector
- fv-zmad z-axis stepping motor, .05 um resolution
- hbo100w/2 mercury burner (8229)
- pc: p3-1ghz, 512mb ram, viewsonic 22" monitor
- fvx-uvt-bx antivibration platform
lens:
- 100x uplfl plan fl na 1.3, wd .1 (ub535r)
- 60x planapo na 1.4 oil
- 40 x uplanfl na 0.75
lasers:
- fv-lharb: argon laser head (10 mw 488nm)
- fv-lpar: ar power supply
- fv-lhheg: green hene laser head 05-lgp-193
- fv-lhher: red hene laser head 05-lhp-991
- fv-lphe: hene laser power supply
filter cubes:
- wb:excitation: u-m640, wide blue, 450-480, long barrier pass ba515
- ng: excitation: u-m663, narrow green 530-550, long barrier pass ba590
- nu: excitation: 360-370, long barrier pass ba420
filters:
- fv-605bp: 575-630nm bandpass emission
- fv-660lp: 660nm long pass emission
- fv-530bp: fvx-ba 510-530 band pass emission
